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Market chief in temperature and environmental-controlled microscopy, Linkam, offered its vary of cryo workflow options and pattern characterisation instruments at Microscopy and Microanalysis (M&M) 2022.
Linkam previewed its new cryo plunger – CryoGenium – an thrilling new growth in electron microscopy (EM) grid vitrification. Imaging of organic samples embedded in vitrified ice has turn out to be of nice curiosity in recent times because it gives a number of benefits: the organic pattern is in a totally hydrated state with superior preservation all the way down to ultra- structural degree, a vitrified pattern is of course suitable with the vacuum required for EM / single particle (SPT) / correlative gentle and electron microscopy (CLEM), and cryo-fluorescence gives very low photo-bleaching and excessive sign to noise imaging. Preparation and dealing with of vitrified samples usually requires particular abilities and methods. The novel design of the Linkam CryoGenium makes this a easy and reproducible course of. CryoGenium addresses a number of widespread points which are present in a standard plunge- freezing setup. Usually, the pattern is ready on an EM grid and extra liquid is eliminated inside a humidity chamber utilizing blotting paper to regulate the ice thickness of the frozen pattern.
The novel design replaces the widespread blotting mechanism to enhance course of stability and repeatability. The system introduces real-time optical remark of the pattern with management of the liquid movie thickness by adjusting the pace at which the pattern is drawn from the fluid in addition to thinning of the movie thickness by managed suction. The proper situations for plunging may be confirmed by the real-time optical remark and plunging is adopted by automated loading into both a particular cryo-holder or a cryo-transfer container.
CryoGenium gives a platform that will increase the success charge and high quality of plunged grids, enabling improved effectivity and fewer time wasted on the transmission EM (TEM), leading to price financial savings.i
The Linkam CMS196V3Cryo-Correlative Stage gives one other cryo correlative software enabling the total workflow of CLEM to maximise efficiencies within the lab. Cryo-CLEM is a quickly rising method that mixes some great benefits of fluorescence gentle microscopy
(FLM) with three dimensional (3D) cryo-electron tomography (cryo-ET) to disclose the ultrastructure of goal molecules with particular mobile capabilities.ii In addition to sustaining the integrity of the pattern vitrified by liquid nitrogen cooling, the Linkam CMS196V3 stage permits contamination-free pattern dealing with and switch for optical microscopy imaging.
The Linkam vary of pattern characterisation instruments, together with the Modular Drive Stage (MFS), has been particularly designed to achieve a greater understanding of a cloth’s behaviour below totally different temperature, mechanical stress and environmental situations. The techniques provide superior sensitivity, excessive decision and a modular idea for custom-made experiment design and management.
“Supplies science is an thrilling, fast-paced discipline, and it’s important that we discover in depth the efficiency and potential of rising new supplies to unlock their optimistic influence on human life. As scientists search new methods to sort out main international points reminiscent of sustainable vitality and bio-engineering to enhance human healthcare, we have to characterise and perceive the bodily properties of superior supplies. We had been proud to current our newest merchandise at this yr’s Supplies and Microanalysis, underlining our dedication to working in shut partnership with our prospects to develop the know-how that fits the particular necessities of their enterprise.”
Duncan Stacey, gross sales and advertising director at Linkam
To study extra about Linkam, please go to the web site.
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